Proteinase K Powder
Unit Definition
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 umole
(181ug) of tyrosine per minute at pH 7.5 at 37℃.
Features
Appearance: White lyophilized powder
Purity ≥90%
pH: 7.5–12.0 (urea-denatured hemoglobin as substrate), but most often used in pH range 7.5–9.0.
Temperature: maximum activity at 37℃ (In the temperature range of 20–60℃, a minimum of 80% of the activity observed at 37℃ is expected)
Precautions and Disclaimer
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Preparation Instructions
This product is soluble in water (1–2 mg/ml).
Shelf Life & Storage
This product is supplied as a lyophilized powder. Store the product at –20℃.
A Proteinase K solution remains active over a broad pH range (4.0–12.5, optimal pH 8.0) and also over the temperature range of 25–65℃ during use. At pH 8.0, solutions remain active for at least 12 months at 2–8℃. At pH 4–11.5, solutions containing Ca2+ (1–6 mM) are expected to remain active for several weeks. An 80% ammonium sulfate suspension stored at 2–8℃ remains active for at least 12 months.
